Abstract: The biosynthesis of silver nanoparticles is a cost effective and environmental friendly alternative to chemical and physical methods. In the present study, we have studied the green synthesis of silver nanoparticles from T. involucrata leaf extract and their biological properties. Silver nano particles (AgNPs) were characterized using UV-Visible spectrophotometer (UV-VIS), scanning electron microscope, X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR). The AgNPs were tested for cytotoxic, antiangiogenic, antimicrobial as well as DNA diffusion assays. These biogenic nanoparticles showed significant cytotoxic activity against MOLT-4 cell lines, antiangiogenic and antimicrobial activity. Diffusion of DNA was comparatively higher in AgNPs treated cells than the control. Hence, T. involucrata leaf extract mediated AgNPs can be exploited in the development of novel drug. The unique properties of this AgNPs can be put to great use for human betterment.