Abstract: Background and Objective: Medicinal plants are often used in mixture (concoctions) for treatment of diseases in traditional medicine. The study aimed at the determination of the effect of concoction extraction of the leaves Barleria dinteri , Grewia flava and Jatropha lagarinthoides on inherent antioxidant and antibacterial activities. Materials and Methods: The samples of dried ground leaves of B. dinteri , G. flava and J. lagarinthoides were sequentially extracted with n-hexane, dichloromethane, acetone and methanol for both single plant and concoction (mixture of the three plant samples) extractions. The antioxidant and antibacterial activities of the extracts were evaluated through DPPH assay and micro-dilution procedure, respectively with the recording of the EC50 (for antioxidant activity) and MIC (for antibacterial activity) values. Obtained values were statistically compared using student t-test (SPSS). Results: Extracts obtained with polar solvents, acetone and methanol had higher amounts of total phenolic content. Plants extracts had good antioxidant activity with lower EC50 values with the methanol extract of the plant mixture having even much lower EC50 value (0.079 mg mL–1) than single plant extracts. The plants extracts also indicated potential antibacterial activity demonstrated by MIC values less than 1 mg mL–1. High potential antibacterial activity was seen in plant mixture extracts with MIC values ranging between 0.023 and 0.188 mg mL–1. Conclusion: Generally, the EC50 and MIC values of the extracts obtained through concoction extraction were lower than of those obtained by single plant extraction. The results of the current study suggested that concoction extraction of leaves of B. dinteri , G. flava and J. lagarinthoides have enhancing effect on inherent antioxidant and antibacterial activities.