Abstract: Background: Lead is potential environmental contaminants with the capability of causing human health problems and occurred in Egyptian food stuffs. Rosemary is known medically for its powerful antioxidant activity. So, the rosemary extract have been carried out to use as an antioxidant agent against lead hazards in rats. Materials and Methods: The rats divided into six groups including the control group, group 2 received lead solution, group 3 and 4 received rosemary at two doses are 100 and 200 μL, group 5 and 6 received lead plus rosemary at two mentioned doses. At the end of the experiment blood, liver and kidney tissues were taken for biochemical blood analysis and histological examination. Results: The obtained results indicated that lead induced negative effect on all biochemical tested parameters to be 78, 68 and 129 U L–1, 0.55, 3.8, 86, 135, 27, 32, 53, 0.41 and 2.9 mg dL–1 for AST, ALT, ALP, Bil., Alb, Ch., TG, HDL, LDL, urea, Cr and UA, respectively while, the same parameters were in normal value for control or groups received rosemary at two doses. It is worthy to report that the biochemical parameters of groups received lead plus rosemary extract at two mentioned doses were in normal value and close to control value. Histological confirmation was carried out and cleared almost the safety of liver and kidney tissues for all groups except the lead group. Conclusion: It could be concluded that the rosemary extract has the ability to protect against a lead hazard in rats.