Abstract: Background and Objective: The genus Hymenaea August 6, 2016(Fabaceae, Caesalpinioideae) is comprised of 14 species, 12 of which occur in several Brazilian regions commonly known as "Jatoba". Several scientific studies of these species are being conducted to demonstrate their potential for pharmaceutical and food industries. Methodology: This study assessed antioxidant activity and sun protection by several parts of two endemic medicinal plants from Brazil, Hymenaea courbaril and Hymenaea stigonocarpa, through DPPH, FRAP and ORAC-FL assays. Fractionation of 70% ethanolic seed extract from H. courbaril was performed. The flavonoids profile by HPLC-PAD was performed with 70% ethanol fractions that showed better results for antioxidants tests and total phenols content. Results: All extracts showed antioxidant activity and high phenolic compound content, except the pulp extract. The fraction ethyl acetate methanol (EAM) 70:30 showed better results for DPPH, FRAP and total phenolic content (72.21±1.08%, 3029.97±09.78 μM TE g–1 extract and 491.56±02.62 mg GAE g–1 of dry extract, respectively). The HPLC-PAD analysis revealed a spectrum with typical absorption bands of flavonoids for the fraction ethyl acetate:methanol 70:30. The results of this study indicate that the 70% ethanol extract from seeds of the specie H. courbaril and its fraction EAM 70:30 showed high antioxidant activity, as well as the presence of phenolic compounds. Conclusion: These results suggest that H. courbaril should be further explored with a focus on use in cosmetic, pharmaceutical and food formulations.