Abstract: The synthesis, characterization and application of biologically synthesized nanomaterials have become the prime area of study in nanotechnology. Green synthesis of nanoparticles using plant extracts is being explored globally owing to the absence of disadvantages associated with conventional methods. This study reports the synthesis of silver nanoparticles using the extract of Tridax procumbens Linn. leaves, characterization of the synthesized nanoparticles and the evaluation of blood clotting activity of the nanoparticles. Ultraviolet-Visible (UV-Vis) spectroscopy confirmed the synthesis of nanoparticles. X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) studies revealed the characteristics of the nanoparticles synthesized. The nanoparticles have been found to reduce the time taken for blood clotting significantly (p = 0.01) and thus proved to be efficient hemostatic agents. Tridax procumbens leaves exhibit hemostatic activity by promoting blood clotting. The nanoparticles synthesized using extract of the leaves of T. procumbens were found to promote blood coagulation better than the plant extract. The hemostatic potential of the nanoparticles can be further validated in vivo.