Abstract: Black pepper (Piper nigrum L.), known as the king of spices, proven to contain various types of active substances that are allegedly beneficial to the human body functions including sexual function. This study was conducted to determine whether black pepper extract can be used to improve sexual function in male subjects. Thirty six healthy male mice, four months old, weighing between 25-30 g, were used as experimental animals and grouped into four. The first one mice was given pellets that do not contain black pepper extract as a control. The second and third groups, respectively were treated with pellets containing aqueous and ethanol extracts. The last group was given pellets containing aqueous extract and ethanolic extracts with a ratio of 1:1. Pellets were given once every day for 90 days. Mating behavior test was conducted in an open round plastic tray with a diameter of 40 cm and height 25 cm. Throughout the test, videotaping was performed for the following parameters: courtship latency and mounting frequency. The results revealed that compared with control group, male mice fed on aqueous as well as ethanol extract of black pepper significantly showed a shorter courtship latency (p<0.05). On the other hand, there was no difference in the mounting frequency between treated groups. Interestingly, the mounting frequency showed a strong negative correlation with the courtship latency (r = -0.968). In conclusion, the fruit extract of black pepper potentially affect sexual drive in male mice.