Abstract: Rambutan honey has been reported to be effective therapeutic in wound healing, may be due to it antioxidant activity, but research of rambutan honey antioxidant activity is still very limited. The purpose of this study is to determine rambutan honey free radicals scavenging activity in vitro and lipid peroxidation inhibition of oral mucosa wound tissue in vivo. The research method was experimental laboratory. The sample used for DPPH test was pure isolated rambutan honey with concentration of 0.5, 1, 2, 4 and 8 mg mL–1. Experimental animals used were 64 male Wistar rats. All rats were injured except for the Negative Control group (NC), using the 4 mm-diameter punch biopsy on the palate and then divided into groups of Positive Control (PC), a group with a topical application isolate of Rambutan (RH) 1 mL and group with topical application of Vitamin C (VC) 0.1 mL, then sacrificed at day 0, 3, 7 and 14 and measured lipid peroxidation inhibition of wound tissue with TBARs method. Data were analyzed statistically, using Kruskal Wallis and Mann-Whitney post hoc. The results showed rambutan honey 1 mg mL–1 have DPPH inhibition of 45.3% and the lipid peroxidation inhibition activity in oral mucosa wound tissue significantly on day 3 (p = 0.028) and 14th (p = 0.037). The conclusions were rambutan honey has antioxidant activity in vitro and in vivo that these natural substances have the potential to be used as an antioxidant drugs in human oral mucosal healing.