Abstract: Extracts in organic solvents (namely petroleum sprit, dichloromethane, ethyl acetate and methanol) of one of the important medicinal plants- Lippia alba were evaluated for their antibacterial activities against five pathogenic bacteria such as Bacillus subtilis, Sarcina lutea, Xanthomonas campestris, Escherichia coli and Klebsiella pneumoniae by estimating zones of inhibition as produced by disc-diffusion method on Nutrient agar medium. All the bacteria showed susceptibility against the petroleum sprit and ethyl acetate extracts, whereas, the dichloromethane and methanol extract showed least activities. It was found that antibacterial activity of the petroleum sprit extract showed maximum zone of inhibition against S. lutea (22 mm) followed by X. campestris (14 mm) B. subtilis (12 mm) and E. coli (12 mm). Ethyl acetate extracts showed highest activity 12 mm zone of inhibition against both S. lutea and X. campestris. The minimum inhibitory concentration of petroleum sprit and ethyl acetate extracts were ranged between 32-256 μg mL-1 for tested bacteria. This study demonstrates the potential of Lippia alba as a source of antibacterial agent that could be effectively used for future health care purposes.