Abstract: Plants have been exploited to a large extent for the treatment of human diseases in different parts of the world. Plant products with antibacterial properties have obtained enormous emphasis for exploration of its novel bioactive compounds. Underutilized edible fruits of Physalis minima L. have been screened at their successive stage of growth and ripening to identify its antibacterial potential using agar well diffusion method. Various non-polar to polar infusion extracts were used to determine zone of inhibition and minimum inhibitory concentration against medically important bacterial strains namely Bacillus cereus, Bacillus subtilis, Micrococcus luteus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumoniae, Salmonella paratyphi and Salmonella typhi. Methanol and ethyl acetate extracts of mature and ripened fruit showed significant activity against Micrococcus luteus and Bacillus subtilis. The study also demonstrates the influence of maturity indices of P. minima fruit on its antibacterial potential and demands further studies to identify the bioactive natural compounds present so as to serve and facilitate pharmacological studies.