Abstract: Ginger has been used in traditional Indian and Chinese medicine and is effective for a wide range of ailments including diarrhea, respiratory disorders, inflammatory diseases, arthritis etc. Recent studies have shown the role of ginger extract in the modulation of biochemical pathways involved in chronic inflammation and have thus provided evidences for its anti-inflammatory property. The aim of the study was to identify and purify a novel protein from ginger rhizomes (Zingiber officinales), of Zingiberaceae family possessing anti-fungal, anti-inflammatory and anti-proliferative properties as elicited using antibiotic diffusion method, surface plasmon resonance, spectrophotometric analysis and flow cytometry, respectively. The purified protein G-24 having molecular mass of 24 kD exhibited a potent anti-fungal activity against the mycelial growth in Fusarium exysporum and Candida albicans. It had shown 60% inhibition of human oral cancer cell line (KB cells) at 10 μM concentration. It inhibited inflammatory enzymes; lipooxygenase (LOX) and cyclooxygenase-2 (COX-2) with KD values of 2.04 μM and 2.74 nM, respectively. This confirmed the anti-inflammatory property of G-24.Thus, concluded that the G-24 protein possessed multiple functions viz. antifungal, anti-inflammation and antiproliferation.