Abstract: The aim of the present study was to investigate total phenols and flavonoids content of 53 traditionally used medicinal plants of western region of India. The plants/plant parts were extracted by cold percolation method in acetone and methanol. Qualitative phytochemical analysis was done for various phytoconstituents like alkaloids, tannins, cardiac glycosides, steroids and saponins. Total phenol and flavonoid conent was quantitatively estimated. Total phenolic contents were determined by the Folin-Ciocalteus reagent method. Total phenolic content was expressed as GAE (Gallic Acid Equivalents). Total flavonoid content was determined by the aluminium chloride colorimetric method. Total flavonoid content was expressed as quercetin equivalents. Tannins were present in more number of plants followed by cardiac glycosides and steroids. The Mangifera indica showed highest phenolic content, maximum content being in the methanol extract followed by Strychnos nux-vomica, Origanum marjoram, The Aristolochia bracteolate showed highest flavonoid content, maximum content being in the acetone extract followed by Phyllanthus reticulates, Argemone mexicana. Many reports suggest a positive correlation between total phenolic content and antioxidant activity so it can be stated that the Mangifera indica may possess good antioxidant property because of its high phenolic content.