Abstract: Antibacterial properties of different extracts of Chlorophytum borivilianum were studied. Ethanol, ethyl acetate, acetic acid and water were used to prepare the extract. The antibacterial activity of different extracts were carried out against four bacteria, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis, by agar cup diffusion method. Zone of inhibition produced by different extracts were measured. Acetic acid extract of C. borivilianum showed antibacterial activity against all the tested bacteria in the order of sensitivity as Staphylococcus aureus>Pseudomonas aeruginosa>Escherichia coli>Bacillus subtilis. The antibacterial activity of Staphylococcus aureus was sensitive with 6, 24, 12 and 8 mm zone of inhibition at 10 mg mL-1 of water, acetic acid, ethanol and acetone extract respectively. For, Pseudomonas aeruginosa zone of inhibition is 8, 20, 12 and 10 mm for water, acetic acid, ethanol and acetone. Escherichia coli revealed no zone of inhibition for water extract whereas it possess 18, 10, 2 mm zones of inhibition at 10 mg mL-1 for acetic acid, ethanol and acetone respectively. Bacillus subtilis showed 3, 20, 9 and 4 mm zone of inhibition at 10 mg mL-1 for different extracts. These results showed that the extract has a wide range of antibacterial property than the other extracts.