Abstract: The aim of this study was to test the in vitro antiplasmodial effect of extract and different fractions of Diospyros melanoxylan. Diospyros melanoxylon (Barks) are collected from the lower forest of Uttaranchal, which is identified by the local people. Plant materials are known as source of new antimicrobial agents, as a result search has been to discover new antibacterial drugs of plant origin. Six of eight methanolic extracts were found to have significant activity, in vitro anti-plasmodial effect of the water and ethanol extract of IC50 value is ranging from (IC50 = 116±9.19 and IC50 145±140 μg mL-1) and (IC50 = 35±0.0 and IC50 47±4.24 μg mL-1) of D. melanoxylon against chloroquine resistant and susceptible variety of Plasmodium falciparum. Least phytochemicals was observed in case of petroleum ether. These results, so obtained demonstrate the broad spectrum activity of D. melanoxylon bark extracts which may be useful in treatment of various microbial infections. Extracts of three of the four species of Indian Diospyros tested in this study showed antiplasmodial activities, with the best activity shown by D. melanoxylon. This species especially is worthy of further investigation to determine which of its constituents are responsible for the activity.