Abstract: Present study attempts to determine antimicrobial efficacy of Ampucare stored at different conditions by time kill curve studies against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Proteus vulgaris. In all storage conditions, a rapid killing time was achieved by Ampucare. Bacterial count was less than 3 Log10 cfu mL-1 after 6 h of study in all organisms under study. No deviation in pattern of bacterial inhibition was found in all conditions of storage of Ampucare. There was no re growth reported even after exposure for longer time under influence of Ampucare. In conclusion, Ampucare has good antimicrobial activity under all storage conditions of study against E. coli, S. aureus, P. vulgaris and K. pneumoniae.