Abstract: Antimicrobial activities of the crude methanol extract as well as the n-butanol and residual aqueous fractions from the aerial part of Indigofera conferta used in traditional medicine to treat infected wound were investigated using disc diffusion and broth dilution techniques. The extract and the fractions were tested against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli and Candida albicans using Ampiclox as standard antibiotic. The crude methanol extract and the aqueous fraction exhibited activity against all the organisms tested (zones of inhibition 16-34 and 14-31 mm, respectively). The n-butanol fraction showed activity on Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa only (zones of inhibition 14-25 mm). Phytochemical screening on crude extract revealed the presence of tannins, flavonoids and steroids. This study showed that the leaves of Indigofera conferta contains active compounds and its antimicrobial activity justifies its use in traditional medicine.