Abstract: Clonal multiplication of selected superior genotypes of Gmelina arborea is difficult owing to maturity related loss in adventitious root potential which constitutes a serious constraints in their ex situ conservation and mass multiplication for sustainable use. The present study reports a new procedure for optimizing adventitious rhizogenesis through rejuvenation in the parent material taken from mature trees. Miniature bud sprouts (1-1.5 cm long with 1-3 unexpanded leaves) produced on semi-hardwood shoot cuttings taken from 15 year old trees were evaluated in comparison with juvenile seedling cuttings, coppice shoot cuttings and semi hardwood cuttings. The cuttings were administered with a quick dip treatment for 30 sec of water (T1) or 5 mM (T2) and 10 mM IBA (T3) before planting and observations were recorded on various rooting parameters after 3 weeks. The mini-cuttings exhibited significantly superior adventitious rhizogenesis (94%) with about 2 and 4.7 times more rooting than seedling cuttings and semi-hardwood cuttings, respectively. The treatment of 5 mM IBA resulted in significantly superior adventitious rhizogenesis. The findings indicate rejuvenation of parent material in the procedure which may be employed for large-scale clonal multiplication and ex situ conservation of mature ortets of Gmelina arborea having desirable traits.