Abstract: An attempt was made to isolate and to identify the soil microbial populations from the rhizosphere at Pachamalai forest area. This is located in the Eastern Ghats of India. There are 18 species of bacteria, 9 species of fungi and 7 species of actinomycetes were observed and identified in the help of Bergey’s manual and manual of soil fungi they have characterized. This is the first report of kind from Pachamalai forest. These microbes might have played very important role in sustaining in forest ecosystem in Pachamalai forest of Eastern Ghats.