Abstract: A protocol has been developed for regeneration of plantlets from leaf primordial of Aegle marmelos. Aegle marmelos is a middle sized slender aromatic armed tree native to India and distributed South-east Asia. The in vitro plant regeneration studies indicated that the following combinations of hormones with MS media containing Kn (1.5 mg L-1) + 0.5 mg L-1 2, 4-D with NAA (1.0 mg L-1) is most suitable for organogenic calli formation and shoot induction. These calli later developed shoots when transferred to MS medium containing NAA (1.5 mg L-1) with (0.5 mg L-1) IBA for root initiation. Rooted plants survived well under acclimatization.