Abstract: Background and Objective: Pyrethroid insecticides are commonly deployed across the globe to combat grape pests, yet little is known regarding the environmental risk associated with runoff from vineyards. Therefore, the objective was to quantify accumulation, transport, and environmental fate of a common-use pyrethroid (bifenthrin) administered to concord grapes in a research vineyard in northwestern Pennsylvania. Materials and Methods: Bifenthrin was applied to concord grape vines at three recommended application rates three times during the growing season (July, August, September), with soil samples collected at one- and 14-days post-spray. Using 24 h single-point Tenax extractions, the bioaccessible fraction of bifenthrin in soil samples was analyzed. Furthermore, the potential risk of runoff and transport into local waterbodies was modeled. To validate the results of the model, sediment and water samples were collected from three local streams in close proximity to vineyards. Results: Bifenthrin in field-collected soils increased over the growing season, with higher detection frequencies in rows with higher application rates. While the model predicted runoff to adjacent water bodies and potential toxicity to non-target species, no bifenthrin was detected in field-collected stream samples (water and sediment). Conclusion: The hydrology of local streams likely protects from bifenthrin accumulation, thus reducing the environmental risk of pyrethroid use in Pennsylvania vineyards.