Abstract: Background and Objective: Increased milling of oil palm in the South-East area of Nigeria has led to copious production of palm oil mill effluents (POME) whose disposal on agricultural farmlands has brought unforeseen challenges. If this POME is disposed, is it fertile or toxic? What are its constituents and chances of appropriate disposal in water body? In this study, the effect of palm oil mill effluent (POME) on the integrity of soil was investigated. Materials and Methods: The samples (POME and soil) were collected from ROCHE-ADAPALM milling site in Ohaji-Egbema LGA, Imo state. Soil samples from the POME dumpsite as well as a non-POME site, both at different depths and were assessed for their enzymatic and physicochemical properties using standard analytical procedures. Results: The results of the physicochemical analysis of the POME were obtained in the following range: pH, 5.2-6.3; Dissolved Oxygen (DO), 12.20-12.60 mg L–1 Biochemical oxygen demand (BOD), 120-130 mg L–1 Chemical oxygen demand (COD), 488-504 mg L–1. Findings revealed that the enzyme activities were affected significantly (p<0.05) with differing levels of activity at both top and subsoil levels. The carbon (%) and organic matter, total nitrogen and water holding contents across top soils showed a significant increase in their respective values with a significant decrease (p<0.05) across subsoils. Conclusion: Indiscriminate disposal of POME on land poses a threat to the soil properties, microbes, plants and by extension the ecosystem. For an entirely stable ecosystem waste materials should undergo quality control as to curtail its excesses.