Abstract: There is a growing concern on the safety of frozen fish imported from various parts of the world where the levels of contamination of water bodies may not be well ascertained and regulatory measures not strictly adhered to. Concentrations of four heavy metals (Lead (Pb), Cadmium (Cd), Copper (Cu) and Mercury (Hg)) in the muscle, gill and liver were investigated for three most consumed frozen fish species (Umbrina canosai, Clupea harengu and Scomber scombrus) within Ibadan, metropolis. Samples were digested as described by FAO/SIDA. The concentrations of metals were quantified using a Varian AA240 Fast Sequential Flame Atomic Absorption Spectrophotometer (AAS). Heavy metals like Cd, Cu and Hg were significantly higher (p<0.05) in the liver of the three species than in the muscle and gills. There was no significant difference in the Pb concentration for the three organs in S. scombrus, whereas, Pb was significantly higher in the liver of U. canosai (9.73 mg kg–1) and C. harengus (4.40 mg kg–1) than in the muscle (6.92 and 2.11 mg kg–1, respectively) and gill (8.73 and 2.52 mg kg–1, respectively). Copper concentration was marginally higher in S. scombrus (5.06 mg kg–1) with the least value recorded in U. canosai (3.28 mg kg–1). However, no significant variation (p>0.05) was observed in the muscle Cd, Cu and Hg levels for the three species. Comparatively, S. scombrus has the highest concentration of heavy metals. This study revealed that the heavy metals investigated in the major organs and flesh was all above the limits recommended by World Health Organization.