Abstract: This study evaluated the mutual relationship among arsenic, iron, manganese, total nitrogen and total organic carbon in the soil of arsenic affected area, Singair, Bangladesh and the results were compared with the other arsenic contaminated area in Bangladesh. The present investigation revealed that high concentration of iron (9138-22071 mg kg-1) and manganese (22-80 mg kg-1) were accompanied by the high concentration of arsenic (44-60 mg kg-1) in arsenic polluted Singair area. In addition, the study showed that the soil of Singair (15-20 m) contained very high concentrations of total organic carbon ranged from 23100-42900 mg kg-1 and the content of total nitrogen was also very significant in some soil samples, where the concentration of arsenic was high. Therefore, the research study can conclude that presence of total organic carbon, total nitrogen, iron and manganese has significant relationship with arsenic contamination in the soil of Bangladesh. However, the study found no significant relationship with arsenic and carbon nitrogen ratio in the soil of Singair.