Abstract: The present study deals with the interaction between accumulation of waterborne lead and chromium in Labeo rohita fingerlings in the laboratory. There was no mortality occurred in individual and binary treatment. Kidney, liver, gill, skin and muscle of L. rohita exposed to sublethal concentration 0.10 μg Pb L-1 alone and 0.10 μg Pb L-1+0.15 μg Cr L-1 accumulated Pb linearly with time over 90 days and significant differences occurred with Pb alone. Lead accumulation in binary mixture Pb+Cr significantly decreases than Pb alone in all tissues of fish. So, the interactive effect of Cr on Pb accumulation was of antagonistic in nature. Cr is accumulated linearly with time in Kidney, liver and gill of L. rohita exposed to 0.15 μg Cr L-1 alone and 0.10 μg Pb L-1+0.15 μg Cr L-1 for 90 days. Muscle reached a steady state Cr levels after 60 days of exposure. The interactive effect of Pb on Cr accumulation was synergistic in nature in Pb+Cr mixture. The presence of Cr in a mixture decreases the accumulation of Pb. Though kidney, liver and gill tissues of L. rohita accumulated Pb and Cr above permissible level but muscle accumulates below human consumption level.