Abstract: The primary objective of the present study was to evaluate the polluting sources and inflow of the Shoteit River and offer several practical solutions to reduce such contaminations. Therefore, it is essential to determine the polluting load of the inflowing wastes to the River. The polluting sources of Shoteit River can be divided into three sectors including agricultural, industrial and urban. Contaminants were identified and the polluting load was calculated accordingly. The total annual pollution load flowing into Shoteit River from the cities of Shushtar and Gotvand includes 1539 t of BOD5, 4493 t of COD and 8071 t of TDS. The result of present study, as expected, showed that most pollution of Shoteit River environment is caused by agricultural wastes having a higher discharge compared to the wastes produced by the two other sectors. Industrial polluting sources have the second largest polluting load to the Shoteit River. The urban polluting sources are in the third position. It should also be noted that solid wastes affect the pollution state of this River. After analyzing the data, several practical solutions are proposed to alleviate the problems caused by polluting inflow to the River. Alternatively, constructed wetlands can be used for primary treatment, where the wetland is the only type of treatment used and in this case, toxic effects on the aquatic plants due to the high organic loading of the influents have been reported.