Abstract: In this study, the dust (total) at exterior surfaces of windows and street dust (<63 μm) in the vicinity of both busy and quiet streets were sampled in order to determine the concentration of anionic and cationic surfactants, as well as anions (sulfate, nitrate and chloride) to indicate its possible sources. The sampling locations were Bandar Baru Bangi, Bandar Kajang and Seremban, which were selected due to the traffic density in those areas. Samples obtained were analyzed by caolorimetric methods using Methylene Blue Active Substances (MBAS) for anionic surfactants and Disulphine Blue Active Substances (DBAS) for cationic surfactants. The results obtained indicate that the concentration of surfactants was higher in busy areas for both windows and street dust in comparison to quiet areas; although, the difference noted was insignificant (p>0.05). Such that, it is suggested that combustion in car engines is mostly likely to be the source of surfactants in both areas. Additionally, the positive correlation recorded between surfactant and nitrate concentration (R2nitrate = 0.51) added further support to surfactants mainly being produced as a result of vehicular emissions. On the other hand, the insignificant correlation between both surfactants in street dust and on windows in busy areas suggests that the presence of surfactants originated from different sources.