Abstract: Background and Objective: Polluted water in a natural recreation river can cause an adverse effect on human health if it is mistakenly consumed by the users. Bacteria presence with the group of Enterobacteriaceae, like an E. coli survived well in the environment and is often used as an indicator for water contamination. The main aim of this study was to determine the presence of E. coli bacteria in a famous water recreation Park in Malaysia via soil and water sampling. Materials and Methods: The study was carried out during a student’s practical visit in May, 2019 at a recreation waterpark at Hulu Langat, Selangor. Both water sample and soil samples were collected from a few regions at the recreation park for E. coli investigation. Next, test like BOD estimation and colilert test was carried out in the university laboratory to detect the presence of E. coli bacteria. Results: The entire water sample collected from the upper, mid and lower regions was positive in colilert test, which indicated the presence of E. coli bacteria. The BOD reading was above 3, which showed high pollution rate. For the soil testing, an E. coli bacterium was found in the EMB agar. Conclusion: Therefore, awareness of the potential risk associated with this recreation water park needs to be communicated with the public and users should not contaminate the water and surrounding areas while visiting.