Abstract: Background and Objective: Flowering calendar of a particular area will give the information of the period and duration of flowering among plants. The purpose of the study is to investigate the presence of the habitat of some dominant macrophytes in Keibul Lamjao National Park (a Ramsar site) which have ethnobotanical relationships with human beings (the lake dwellers in particular and tourist in general) and wild animals. Materials and Methods: Flowering calendar of 6 study sites viz., Keibul, Nongmaikhong, Kumbi, Khordak, Sargam and Toyaching were studied and monthly flowering calendars were taken for 2 years period (2010-2011). Flowering calendars of the macrophytes were surveyed by following standard methodologies and comparison of the stages of flowering period of 6 study sites was incorporated. Results: Maximum number of flowering plants was recorded in Sargam (44), Khordak (32), Kumbi (30) and Nongmaikhong (29) during the period September-November. Whereas, Toyaching reached 35, Keibul recorded lowest plant numbers 14 during the period June-August. Four plant species viz., Ageratum conyzoides, Alternanthera philoxeroides, Leersia hexandra and Hedychium coronarium were found throughout the year in all the 6 sites. Conclusion: From the findings, it can be concluded that flowering calendar will give the information of plants which are used as fodder for the wild animals like sangai (Rucervus eldii eldii McClelland) an endangered deer species in this globe and ethnobotanically important plants of the lake dwellers as food, medicinal plants and allergens, thereby maintaining health status of man and animals. Conservation is required by controlling the human anthropogenic harassments, thereby maintaining the luxuriant growth of the seasonal and perennial, macrophytes observed during the study of flowering calendar, so as to maintain and conserve the natural flora and fauna of the park.