Abstract: Background and Objective: Sugar beet is one of the cash crops grown in temperate and cold zones of the world. In Egypt, it thrives in the northern part of the country. It comprises about 30% of total sugar production. Both foliage and pulps are feed of high value for the domestic animals. Since several soil-borne fungi attack this crop causing a significant reduction in the production, the present investigation aimed to evaluate the effectiveness of selected antioxidants including GAWDA formulation along with Trichoderma fungus and organic fertilizers (compost) to induce resistance against the root rot disease along with their possible role on increasing the yield and quality. Materials and Methods: In vitro studies were carried out to select the efficient concentration of the tested antioxidants on reducing the growth of the target pathogens. In vivo studies were applied in the field plots at Mansoura University Campus during the two successful seasons of 2015 and 2016. The growth parameters as well as the root weight and their quality characters were recorded. Data were statistically analyzed by CoStat 6.3 software of analysis of variance at p<0.05 as outlined by Duncan. Results: Soaking seeds in water solution of GAWDA® formulation at a concentration of 4 g L–1 for 12 hours before planting and air dried for 1 h followed by coating them with Trichoderma harazianum (T. harazianum) before planting in soil supplemented with the composted agricultural residues significantly decreased the damping-off disease and scaled up the yield and quality. Conclusion: Results addresses a ramping up in the yield and quality of sugar beet as well as control damping-off disease when treated with friendly environmental materials.