Abstract: This study investigates the ability of two bioagents namely Trichoderma harzianum and Streptomyces viridosporus to induce elevated levels of resistance in two susceptible genotypes Morocco and Sids-1 against leaf rust disease of wheat. Bioagents were tested at seedling and adult stage of two susceptible wheat genotypes (Morocco and Sids-1) under greenhouse and open field conditions in 2013/2014 growing season. It was indicated that all bioagents and combined effect between them, increased both Incubation Period (IP) and Latent Period (LP) of the disease and decreased the number of pustule cm–2, than the control. The results revealed that the amounts of photosynthetic pigments, chlorophyll a, b were lower in the infected plants than in healthy ones. It was, also indicated that spraying the plants of both the genotypes with the tested bioagents decreased leaf rust severity (%), increased spike weight (g), grains weight/spike (g) and 1000 kernel weight (g) significantly, as compared with control.