Abstract: This study was carried out in order to evaluate effect of treating soybean seed (Glycin max L.) as seed coating with fungicidal (Vetavax-thiram 200), seed dressing with biotic (Bio-arec, Bio-zeid) and seed soaking with abiotic (Salicylic acid) in controlling fungal diseases and improving seed and seedlings vigor of cultivars (Giza 21, G.35, G.111, G.22, G.82, Klark, Crawford) . The obtained results showed that, Alternaria spp., Aspergillus spp., Cercospora kikuchii, Diaporthe sojae, Fusarium moniliforme, F. semitectum, F. solani, F. oxysporum, Macrophomina phaseolina, Rhizoctonia solani and Penicillium spp., were associated with soybean seed with different percentage. All treatments gave high significant p≤0.05 protection against seed-borne fungi in seed and seedling health evaluation test when applied seed treatments compared with control. Vitavax-200 was the most effective where lead to eliminated the mentioned fungi completely and improve of seed and seedling vigor characters. Giza 21 cv. recorded the less value borne of total fungi as compared of cultivars. Biotic reduced numbers of fungi and gave protection against pathogenic fungi and increased values seed and seedling vigor of soybean cultivars. Giza 82 cv. showing better seed and seedling vigor and less seed infection by pathogenic fungi in seedling health evaluation test. Fusarium moniliforme has been widely and most frequently with seed rot followed by F. semitectum. Fusarium solani was responsible for greater proportion of abnormal seedlings followed by F. oxysporum. Abiotic salicylic acid played important role in enhancing seed vigor, increased seedling length, dry weight of seedlings, reduced number of total fungi on seed and diseases incidence in seedlings (abnormal seedlings, seed rot).