Abstract: Experiment was conducted at BHU in the year 2012-13. Radial growth was not significantly different for most of the isolates. Seven DAI (days after inoculation) highest radial growth has obtained in isolate RF-1 (35.50 mm), Ten DAI maximum growth was observed in isolate EC-1 (52.00 mm) and Thirteen DAI maximum radial growth was same observed in two isolates IIVR and BHU-1 (88.75 mm). The maximum mean mycelial growth was observed in isolate IIVR (57.83 mm) followed by MF-4 (57.66 mm) and BHU-1 (56.83 mm). Isolates of A. solani depicted high variability in pigment production on PDA medium. Mycelial growth patterns were observed on PDA where BG RF-1 SF-1 MF-4 BHU-1 grew with circular margin with smooth surfaced colony and AF-2, PN-4, EC-1, BX-2 and IIVR isolates grew with irregular margin and rough surface. Five varieties of tomato were screened with 10 different isolates. The variety Selection-7 and H-86 were highly susceptible and susceptible, respectively with all the isolates tested. The variety Feb-2 was resistant while, the variety Flora Dade and Swarna Naveen were highly resistant with all the isolates. There was no significant difference between Flora Dade and Swarna Naveen. Out of 10 isolates, only three major groups were recorded on the basis of SAS analysis likewise Group-A isolates were highly virulent (MF-4 and PN-4), Group-C isolates indicate virulent (BG, AF-2, EC-1 and RF-1) and Group- E isolates were less virulent (BHU-1, IIVR, SF-1 and BX-2) with all the five varieties.