Abstract: Fusarium graminearum schwabe and Fusarium moniliforme sheld, conidia germination were controlled by using medicinal plant leaf extracts under in vitro condition. Totally 46 plant leaf extracts were used for the screening purpose. Among them, zimmu leaf extracts recorded minimum of 15 and 18% germination of F. graminearum and F. moniliforme conidia. The screened top four plant leaf extracts were tested against the seed-borne Fusarium sp. by poison food technique under different concentrations (0.1, 0.2 and 0.5%). Among the four plant extracts 0.2% zimmu leaf extract recorded maximum inhibition of seed-borne Fusarium sp. mycelial growth, both ethanol and methanol extracts. Among the different concentrations 100% recorded the maximum inhibition area of 6.160 cm2 against F. graminearum and F. moniliforme. It was for determining best concentration to further development of Emulsifiable Concentration (EC) formulation for field trial.