Abstract: Blister bark caused by Trichosporium vesiculosum Butl. is a lethal fungal disease of Casuarina equisetifolia Forst. In plantations, blister bark is more prevalent in trees that are more than two and a half years old. So in this present study, the influence of climatic and edaphic factors on blister bark disease development in C. equisetifolia under controlled conditions were investigated. The pathogen isolated from the disease plant tissues was maintained on Potato Dextrose Agar medium. Conidial suspension of the pathogen was inoculated onto 3 to 6 months old. C. equisetifolia seedlings raised on different soil types (Alfisol, Vertisol and Ultisol). The pathogenecity study indicated that T. vesiculosum attacked C. equisetifolia seedlings as early as 5 months old. A temperature range of 25 to 30°C and a relative humidity (%) range of 45 to 60 favoured the conidial development and fungal spread in the seedlings. Similarly soil pH of 5.5 to 6 favoured the pathogen and disease development whereas soil pH<7.5 deters disease development. It was also found that regular watering prevented disease development. Soil type seems to play a major role in disease development as disease incidence and severity was more in seedlings raised in Vertisol than in other soil types. From this experiment it was understood that the favourable temperature, soil factors and soil type for this pathogen to be taken care at the time of planting of C. equisetifolia seedlings so as to avoid the blister bark disease.