Abstract: In this research we used Turnip vein clearing virus (TVCV) to study viral non-transmission in A. thaliana as a step toward developing a management strategy against seed transmissible viruses. Confocal laser scanning, light, transmission and scanning electron microscopy and immunolabeling techniques were used to study the pattern of the viral movement in reproductive parts of the systemically infected plant. The virus was located in all parts of the flower except ovules and pollen grains. The viral movement was thus confined to the junction of ovule and ovule stalk in the siliques and outside the pollen grains in anther tissue. Subcellular localization of (1 > 3)- β -glucan (callose) indicated selective deposition of this substance in plasmodesmata at the junction of ovule and ovule stalk as well as exine layer of pollen grains. The callose deposition was not observed in uninoculated and mock inoculated control plants. We propose that the observed (1 > 3)- β -glucan may be involved in blockage of viral movement into ovules and pollen grains and result possibly in non-seed transmission of TVCV in A. thaliana.