Abstract: A method suitable for detecting the slow growing seed-borne fungi of peanut was developed. Twenty-five seed samples collected from commercial markets in Egypt were used in this investigation. With the Standard Moistened Blotter Method (SBM) and Deep-Freezing Blotter method (DFB) recommended by the International Seed Testing Association, saprophytes developed quickly and often impaired the detection of parasitic fungi and inhibited the germination of some important seed-borne fungi. Moistening the blotter disks used for seed germination with an alkaline solution at pH 12.5 using NaOH (0.8%) or KOH (0.4%) enhanced the growth and recovery of the slow growing seed-borne pathogens Cephalosporium sp. and Verticillium sp. The treatments also were effective in suppressing the growth of saprophytes which impair the detection of pathogenic fungi on seed. We recommend using the alkaline blotter method for seed health testing when searching for slow growing seed-borne fungi.