Abstract: Ability of biocontrol agents, fluorescent Pseudomonas, non-pathogen Fusarium strain and Trichoderma harzianum T-22 applied in combination and alone, to control of Fusarium oxysporum f. sp. lycopersici was studied in the greenhouse. Tomato roots were treated with biomass of flourescens Pseudomonas strain from rhizosphere, non-pathogen Fuasrium sp. and Trichoderma harzianum T-22 individually and in combination and planted in artificially infested soil with pathogen F. oxysporum f. sp. lycopersici. Although all biocontrol agents applied individually reduced disease incidence, treatments as combination, except for nonpathogen Fusarium strain + T. harzianum T-22, showed more protective effect. Combination of T. harzianum T-22 + fluorescent Pseudomonas isolate gave the best control (70.2%).