Abstract: Twenty isolates of fluorescent Pseudomonads were isolated from pathogen suppressive soils of pigeonpea fields. Thirteen isolates were identified as Pseudomonas aeruginosa, 5 as P. fluorescens while remaining 2 needs characterization Pseudomonas sp. Four isolates (Pf604, Pf605, Pf611 and Pa616) were considered potential for the biocontrol of the disease on the basis of antibiotics sensitivity, antifungal activity, fluorescence produced by Pseudomonas, inhibitory effect on the hatching and penetration of nematodes and colonization of pigeonpea roots by these isolates. Evaluation of these isolates under pot condition revealed that isolate Pf605 was the best biocontrol agent for the wilt disease complex followed by Pf604, Pf611 and Pa616. Isolate Pf605 caused greater reduction in nematode multiplication and also reduced wilt index under pot condition.