Abstract: Begomoviruses represent one of the major groups of phytopathogens of many economically important crops worldwide. In Trinidad, Potato yellow mosaic trinidad virus (PYMTV) has been implicated as the causal agent of disease epidemics in tomato since the late 1980’s. Surveys of dicotyledonous weeds found in commercial tomato cultivations in addition to weeds found in non-tomato-growing areas were conducted to determine possible PYMTV inoculum sources other than tomato. Detection of viruses was confirmed through dot blot hybridization and immunocapture-PCR (IC-PCR) amplification. None of the weeds tested in the study were hosts of PYMTV. However, two weed species were found to be naturally infected with two distinct begomoviruses as confirmed through partial nucleotide sequence comparisons. There was also no evidence of mixed begomovirus infections.