Abstract: A survey was conducted to determine incidence, prevalence and inoculum density of Verticillium dahliae, the causal agent of Verticillium Wilt (VW) of cotton, in southern Turkey. Survey of VW in 151 cotton fields from 30 locations of Hatay, Kahramanmaraş, Adana, Mersin and Osmaniye provinces in 2000 and 2001 indicated that the disease occurred in 37,1% of the fields. The mean disease incidence of plants with vascular discoloration was 31.2%, the mean severity of the disease was 0.9 ranging from 0.1 to 3.4 (according to 0 to 4 scale) in the region. Analyses of soil samples by wet sieving method revealed that the mean inoculum density ranged from 7 to 28.4 microsclerotia (ms) g-1 of soil in the region.