Abstract: Evaluation of different strains of Pseudomonas fluorescent for the biological control of chickpea wilt shows that among 36 rhizobacterial isolates, Isolate 1, Isolate 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 and 36, during in vitro studies, the isolate No. 3, 6, 12, 16, 18, 21, 22, 23, 27, 29 and 33 shows antagonistic activity during inhibition of Fusarium oxysporum f.sp. ciceri by rhizobacterial isolates, whereas by using water culture in test tubes the isolate No. 1, 2, 4, 5 and 9 show antagonistic activity by reducing wilt incidence by 91.67, 94.33, 94.37, 96.00 and 96.00% decrease over control, respectively. In try experiment isolate No. 1, 2, 4, 5 and 9 proved to be the antagonistic ones.