Abstract: An experiment was conducted to assess the effect of different inoculation times with MS-6, MS-9 strains of Macrophomina phaseolina and CS-1 strain of Colletotrichum corchori on the seed yield contributing characters and quality of late jute seeds (Corchorus capsularis L). Varieties were BJC-7370 and CVL-1. Results showed that inoculation with the pathogens was conducted on 45,55,65 days age of the jute plants. Variety BJC-7370 and CVL-1 of jute had reaction with strains MS-6, MS-9, and CS-1 at every inoculation times. The gradual increase of inoculation affected the plants. As the time of inoculation increases the lesion size on stem decreases, the number of pods plant-1, seeds plant-1 and Seed germinations (%) increases and at the same time seed infections (%) decreases. MS-6 strain was found more virulent than MS-9 and CS-1 regarding pods plant-1 and seeds plant-1 production, as well as seed germinations and infections(%).