Abstract: In order to identify sources of genetic resistance against charcoal rot disease in urdbean caused by Macrophomina phaseolina (Tassi) Goid, 71 germplasm accessions were evaluated by paper towel technique under Laboratory conditions. It was observed that 6 genotypes (45718, 45719, 45721, 45731, VH9440034-1 and VH9440034-7) were highly resistant, whereas 7 were resistant and 10 were moderately resistant. Sixteen genotypes were tolerant whereas rest of the accessions was susceptible or highly susceptible. The paper towel technique proved to be were and efficient for identification of resistance in urdbean for charcoal rot disease. Resistance observed in this experiment will also be confirmed under field conditions in future.