Abstract: To control the fungal pathogens attacked sugarbeet plant, we subjected R. solani and S. rolfsii to different gamma irradiation doses (0.0, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 and 7.0 kGy for R. solani and 0.0, 0.5, 1.0, 2.0, 3.0 and 4.0 kGy for S. rolfsii). The growth activities of R. solani were completely inhibited at dose 7.0 kGy, while S. rolfsii at 4.0 kGy. The infection percentage was inhibited with gamma irradiation doses increased for both R. solani and S. rolfsii. This was confirmed by the SDS-PAGE for cellular proteins of both R. solani and S. rolfsii, which showed a great variation in protein bands for the same fungus at different gamma irradiation doses. Finally, we can conclude that the gamma irradiation dose to control pathogenic fungi varies with different pathogenic fungi to control the pathogenicity of R. solani, we irradiate it at 7.0 kGy dose while at 4.0 kGy for S. rolfsii.