Abstract: The study was conducted to determine the fate of herbicide (isoproturon) in the grain, straw and soil samples for the control of jangli jai in the wheat fields. Arelon (45% isoproturon) at a dose of 3.7 L ha-1 to control jangli jai was sprayed. The wheat control rating of isoproturon in control and treated plots were observed as highly significant (P<0.05). Similarly, isoproturon application has also been found to be highly significant (P<0.05) in increasing the grain yield of treated plots. The residue concentration in the whole plants of treated plot was 0.66 and 0.71 ppm, 0.25 and 0.26 ppm in grain, 0.37 and 0.36 ppm in wheat straw and the soil collected from treated plots contained 1.74 and 1.76 ppm residue of isoproturon determined by thin layer chromatography and spectrophotometric techniques, respectively. Grain and wheat straw samples from control plots contained no residues but soil samples contained 0.014 and 0.018 ppm of isoproturon.