Abstract: Out of five biocontrol agents/antagonistic microbes viz., Verticillium lecanii, Paecilomyces fumosoroseus, Beauveria bassiana, Cladosporium cladosporiodes and Metarrhizium anisopliae, Beauveria bassiana proved the best in retarding the postulation/rust development in wheats. When the microbes tested alone against leaf rust development, B. bassiana proved excellent and in combination with Verticillium lecanii and Paecilomyces furnosoroseus. Although there was an increase in colony diameter after 2nd week but the pattern of growth was the same as after one week of incubation of bio-control agents application.