Abstract: Background and Objectives: Passion fruit (Passiflora edulis Sims) seeds contain antioxidants and are expected to decrease the levels of cholesterol and malondialdehyde (MDA). The purpose of this study was to identify the effects of a passion fruit seed ethanol extract on Wistar rats fed atherogenic feed. Materials and Methods: The method of this study was a preclinical trial (post-test control group design) in rats by administering passion fruit seed ethanol extract for 14 days, using 26 male rats (Rattus norvegicus) aged two months, divided into 5 groups. The groups were a negative control group (atherogenic feed), a positive control group (standard feed) and test groups that were given the passion fruit seed ethanol extract at doses of 5 mg kg–1 body weight (BW), 10 mg kg–1 BW, or 20 mg kg–1 BW for 14 days to the Wistar rats fed with atherogenic feed. Results: The study results showed a significant difference in MDA levels, which was found in the group that was given passion fruit seed extract(10 mg kg–1 BW)and the positive control group that was given standard feed (mean±standard deviation: 1.83±0.40 mM vs 1.38±0.12 mM; p = 0.002). The level of total cholesterol also showed a significant difference, which was found in the group that was given passion fruit seed extract (10 mg kg–1 BW) with a negative control group that was given atherogenic feed (mean±standard deviation: 84.54±13.69 mg dL–1 vs 68.04±6.17 mg dL–1; p = 0.003). Conclusion: Administration of passion fruit seed extract showed a significant difference in the level of triglycerides, which was found in the negative control group that was given atherogenic feed compared with the group that was given passion fruit seed extract at a dose of 5 mg kg–1 BW (mean±standard deviation: 1.09±0.30 mg dL–1 vs 0.77±0.25 mg dL–1; p = 0.048).