Abstract: Background and objective: The protective action of fruits and vegetables are associated with the presence of anti-oxidants, notably anti-oxidant vitamins, such as ascorbic acid, α-tocopherol and β-carotene. The aim of this research was to measure the nutritional content, mineral composition and antioxidant activities of Muntingia calabura Linn. fruit, which is grown in Makassar, Indonesia. Materials and Methods: Proximate parameters (moisture, ash, protein, carbohydrate and ascorbic acid) were evaluated using the Association of Official Analytical Chemists (AOAC) method, oxalate and phytic acid were evaluated by redox titration method and saponin and alkaloid content were evaluated using the gravimetric method. Mineral analysis was conducted using the inductively coupled plasma-optical emission spectrometry (ICP-OES) technique and antioxidant activity was evaluated by the fluorescence recovery after photobleaching (FRAP) method. Results: The results showed that the fruit of M. calabura had a high content of moisture and ash. The fruit had iron (Fe) as the higher component in trace elements and potassium (K) as the higher component in major elements. The anti-nutrients oxalate, phytate, alkaloids and saponins were detected while alkaloids are the highest content. In addition, from the study, the fruit showed antioxidant activity. Conclusion: Muntingia calabura is easily accessible and contributes remarkably to the amount of nutrient intake in the human diet. Further study is needed to analyze the type of alkaloids contained in the fruit.