Abstract: Background and Objective: Brachiaria spp. is a potential grass that can be cultivated in Indonesia. Grass production and quality can be affected by the planting material. This study aimed to identify the nutrient quality and production of Brachiaria spp. with different planting materials in Yogyakarta, Indonesia. Materials and Methods: The planting materials used in this research were seeds (10 days after germination) and pols. This study used a split-plot design in which the main plot is the variety of Brachiaria (B. brizantha cv. MG5, B. ruziziensis cv. Kennedy and B. decumbens cv. Basilisk) and the subplot is planting material. Each treatment was replicated nine times. The parameters observed in this research were dry matter, organic matter, crude fiber and crude protein, fresh production, dry matter production, organic matter production, dry matter digestibility and organic matter digestibility. Results: The planting material significantly affected (p<0.05) the chemical composition. The use of pols increased the dry matter content (23.10%) but also increased the crude fiber content (30.15%). The planting material did not significantly affect (p>0.05) the production and digestibility of Brachiaria spp. Varieties of Brachiaria spp. showed significant (p<0.05) differences in chemical composition, production and digestibility. B. decumbens cv. Basilisk had the highest proportions of dry matter (24%) and organic matter (87%) and B. ruziziensis cv. Kennedy showed the best result for crude protein content (11.86%). B. decumbens cv. Basilisk showed the best result for production with 6.83 t ha–1 fresh production, 1.69 t ha–1 dry matter production and 1.48 t ha–1 organic matter production. B. ruziziensis cv. Kennedy had the greatest dry matter digestibility with 78.40% and B. decumbens cv. Basilisk had the greatest organic matter digestibility with 82.56%. Conclusion: The use of optimal planting material can increase the quality of chemical composition, production and digestibility of several varieties of Brachiaria spp.