Abstract: Background and Objective: Grasses have relatively few intrinsic toxins. Grasses that contain intrinsic toxins include tropical grasses (oxalate acid). This study examined the nutrient and oxalate acid content of Brachiaria in different species, seasons and botanical fractions in Indonesia. Materials and Methods: The species of Brachiaria used were Brachiaria brizantha, (B. brizantha) Brachiaria decumbens (B. decumbens), Brachiaria ruziziensis (B. ruziziensis) and Brachiaria humidicola (B. humidicola). The botanical fractions considered were the leaf and stem. The oxalate acid content was analyzed using High-Performance Liquid Chromatography (HPLC). The data were analyzed using analysis of variance. Results: It was observed that the oxalate acid content in the dry season was higher than that in the rainy season (p<0.05). The results showed that the leaves of all Brachiaria species had higher dry matter, crude protein and extract ether content than the stem in the dry season (p<0.05). Conclusion: Brachiaria grasses cut in the dry season had higher oxalate content than those cut in the rainy season.