Abstract: Background and Objective: Soybeans are known for their positive influence on the prevention of osteoporosis in postmenopausal women. Soybean sprouts produce bioactive compounds that are better than soybeans. This study aimed to compare the potential of soybean sprouts with soybean and ethinylestradiol on the changes of biomarkers of bone turnover activity in ovariectomized rats (OVX). Materials and Methods: Twenty-five female Sprague-Dawley rats aged 2 months were placed into 5 groups: (i) normal control (without OVX) (N-C); (ii) OVX control (OVX-C); (iii) OVX+ethinylestradiol (30μg kg–1 b.wt.,/day, orally) (OVX-E); (iv) OVX+soybean flour (based on a dose 10μg g–1 b.wt.,/day of isoflavones, orally) (OVX-S); (v) OVX+soybean sprout flour (based on a dose 10μg g–1 b.wt.,/day of isoflavones, orally) (OVX-SS). All groups were treated for 6 weeks and all rats were fed an AIN-93M-based diet. Blood samples were collected before and after treatment for analysis of serum biomarkers of bone turnover and estradiol hormone. Data were analyzed using one-way ANOVA, followed by Duncan’s Multiple-Range (DMR) test. Results: The increase of osteocalcin (OC) and beta-crosslaps (βCTx) in the serum of the OVX-SS group was lower than in the OVX-S group and was the same as the OVX-E group. The OVX-C group experienced the highest increase in OC and βCTx. All groups of OVX rats also experienced a significant decline in estradiol hormone. There was no difference in the decrease in serum estradiol in the OVX-S and OVX-SS groups. Conclusion: The results of the study show that soybean sprout flour consumption provides better inhibition of bone turnover activity than soybeans in ovariectomized rats. The potential of soy and soybean sprouts in estradiol hormone recovery on ovariectomized rats is not different.